The main goal of the Core Facility of 3D printing is to facilitate employees, doctoral students and students access to 3D printing technology that enables the design and production of three-dimensional objects. This technology is not limited to the production of commercially unavailable devices or teaching aids, but also allows you to print models of the patient’s organs created using the pre-procedure MRI / PET scan.
The idea behind the creation of the unit is not only to enable the emergence of technical solutions that are innovative or difficult to obtain commercially due to high cost or long waiting times. The unit also offers substantive and advisory assistance, incl. at the stage of creating a model in 3D modeling software. The facility also provides knowledge on the selection of the appropriate material for printing (prioritizing selected aspects: mechanical strength, chemical properties, biocompatibility or aesthetics).
The studio was established as part of the Research University program.
The mission of the facility is to provide various types of services to members of the University community, with particular emphasis on its priority research areas.
The facility is equipped with the following elements:3D printing articles (pdf file, 307 kB)
The team of the Department of Pharmaceutical Chemistry of the MUG offers metabolic stability tests of drug candidates using the in vitro method, using the microsomal subcellular fraction. In a typical assay, microsomes obtained from human liver play the role of a biotransformation catalyst. The potential drug to be tested is incubated under strictly controlled conditions in the presence of the enzyme preparation and appropriate cofactors. Immediately after the start of incubation and at the defined measuring points (maximum incubation time is 2 hours), a sample is taken and then analyzed using the LC-MS technique. The metabolic half-life is determined on the basis of the obtained chromatograms.
• Research teams dealing with the synthesis of new substances with potential application in medicine
• Innovative pharmaceutical companies
• Selection of enzymatic incubation parameters
• Performing a control to determine the chemical stability under incubation conditions
• Development of the methodology of determination of the submitted compounds using the LC-MS technique
• Determination of the metabolic half-life
• They allow to determine the behavior of a potential drug against the human xenobiotic metabolizing system
• The stability of the candidate drug is a favorable feature due to its interaction with the molecular target
• Metabolic stability is one of the key factors influencing the pharmacokinetics of a drug
• Metabolites formed as a result of biotransformation may have an influence on the therapeutic effect as well as possible side effects
The scheme presented above, including the determination of the metabolic half-life, may be supplemented with additional tests, including:
• Determination of cytochrome P-450 isoenzymes responsible for biotransformation
• Determining the possibility of the formation of reactive metabolites that interact with cellular nucleophiles
• Development of a model of the relationship between the chemical structure and metabolic stability within a given series of compounds
Metabolic stability articles (pdf file, 200 kB)
The bioanalytical section offers the following endogenous substances:
• amino acids by liquid chromatography
• biogenic amines, their precursors and metabolites using both liquid chromatography with mass detection (LC-MS, LC-MS / MS) and capillary electrophoresis (CE).
• steroid hormones and their metabolites using both liquid chromatography with mass detection (LC-MS, LC-MS / MS) and capillary electrophoresis (CE).
• analysis of organic compounds using capillary electrophoresis coupled with LIF detection.
Specification of bioanalytical analyzes performed with LC (pdf file, 707 kB)
Specification of bioanalytical analyzes performed with CE (pdf file, 679 kB)
As part of the analysis of other organic substances, the following tests are performed:
• mass measurement using the ESI technique
• substance identification by “soft” and “hard” fragmentation
• analysis of volatile substances by gas chromatography with mass detection (GC-MS)
• analysis of organic compounds using capillary electrophoresis coupled with LIF detection.
• optimization of the conditions for the separation of analyte mixtures, including the selection of parameters for the preparation of the sample for analysis (extraction), as well as chromatographic and electrophoretic conditions (DryLab, chemometric optimization),
• validation of analytical methods and statistical and chemometric processing of results,
• study of the relationship between the structure and activity (QSAR), chromatographic retention (QSRR) and metabolic stability (QSMSR) of a given series of chemical compounds using chemometric techniques and specialized software: ACD, Dragon, HyperChem, Gaussian, SIMCA
• pharmacokinetic studies in the determination of drugs and their metabolites,
• stability and stability testing of pharmaceutical preparations,
• ELISA tests,
• study of bioavailability and bioequivalence of pharmaceutical preparations.
Szymon Ulenberg, PhD
Department of Pharmaceutical Chemistry
Medical University of Gdańsk
al. Gen. J. Hallera 107
80-416 Gdańsk
+48 602 552 859
szymon.ulenberg@gumed.edu.pl